Cell Imaging and Analysis (CIA) Facilities and Services Core

Core members left to right: Tamara Fraley, Danielle King, Jeff Greenwood (Core Director), Christiane Löhr, and Nancy Kerkvliet

This core supports several of the large cancer prevention experiments through the analysis of tumors by Dr. Loehr. The CIA core has supported the confocal microscopy needs of the Gross and Kioussi labs studying brain and muscle development leading to four recent publications, two in PNAS. In addition, the Greenwood lab, in collaboration with the Mass Spectrometry Core, has determined how phosphoinositides regulate the structure (a Protein Science paper) and proteolysis (JBC paper) of α-actinin in glioblastoma cells. These results have led to a collaborative effort between the CIA and Aquatic Models cores to develop an in vivo model for glioblastoma cell invasion in zebrafish. This model will allow the Greenwood lab to determine the contribution of specific adhesive regulatory mechanisms on glioblastoma cell invasion.

This core merges three separate smaller cores to provide access to key technologies for cell imaging, flow cytometry and cell sorting and the pathological analysis of carcinogenetic studies.

• Cell Imaging and Culture
  
• Flow Cytometry and Cell Sorting
• Tissue Analysis and Pathology

Note: Any manuscript submitted for publication that contains data generated in these facilities should contain an acknowledgement of the services provided by the "Cell Imaging and Analysis Facilities and Services Core of the Environmental Health Sciences Center, Oregon State University, grant number P30 ES00210, National Institute of Environmental Health Sciences, National Institutes of Health."

Publications

Funatake, C.J., Marshall, N.B. and N. I. Kerkvliet (2008). 2,3,7,8-Tetrachlorodibenzo-p-dioxin Alters the Differentiation of Alloreactive CD8+ T Cells Toward a Regulatory T Cell Phenotype by a Mechanism that is Dependent on Aryl Hydrocarbon Receptor in CD4+ T Cells. J. Immunotoxicology (in press).

Siddens, LK, Henderson, MC, VanDyke, JE, Williams, DE and Krueger, SK. (2008). Characterization of mouse flavin-containing monooxygenase transcript levels in lung and liver, and activity of expressed isoforms. Biochem Pharmacol 755:570-579. PMID: 17942081

Chung, WG, Miranda, CL and Maier, CS. (2007). Epigallocatechin gallate (EGCG) potentiates the cytotoxicity of rotenone in neuroblastoma SH-SY5Y cells. Brain Res. 1176:133-42. Epub 2007 Aug 22. PMID: 1790054

Full SI, Deinzer MI, Ho PS and Greenwood JA. (2007). Phosphoinositide binding regulates alpha-actinin CH2 domain structure: analysis by hydrogen/deuterium exchange mass spectrometry. Protein Sci.16(12):2597-604. PMID: 17965186

Golonzhka, O., Leid, M., Indra, G., Indra, A.K. (2007). Expression of COUP-TF-interacting protein 2 (CTIP2) in mouse skin during development and in adulthood. Gene Expr Patterns. Aug;7(7):754-60. Epub 2007 Jun 13.

Pehar M, Vargas MR, Robinson KM, Cassina P, Diaz-Amarilla PI, Hagen TM, Radi R, Barbeito L, Beckman JS. (2007). Mitochondrial superoxide production and nuclear factor erythroid 2-related factor 2 activation in p75 neurotrophin receptor-induced motor neuron apoptosis. J Neurosci. 27(29):7777-7785. PMID: 17634371

Shih, HP, Gross, MK and Kioussi, C. (2007). Cranial muscle defects of Pitx2 mutants result from specification defects in the first branchial arch. Proc Natl Acad Sci U S A 104: 5907-5912. PMID: 17384148

Shih, HP, Gross, MK and Koussi, C (2007). Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expression Patterns 4:441-451. PMID: 17166778

Shih H.P., Gross, M.K. and Kioussi C. (2007). Muscle development: Forming
head and trunk muscles. Acta Histochemica. In Press. PMID: 17945333

Kioussi C, Shih, HP, Loflin, J and Gross, MK (2006). Prediction of active nodes in the transcriptional network of neural tube patterning. Proc Natl Acad Sci U S A 103;18621-18626. PMID: 17132738

Pehar M, Vargas MR, Robinson KM, Cassina P, England P, Beckman JS, Alzari PM, Barbeito L. (2006). Peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons. Free Radic Biol Med. 41(11):1632-44. Epub 2006 Aug 15. PMID: 17145551

Robinson, KM, Janes, MS, Pehar, M, Monette, JS, Ross, MF, Hagen, TM, Murphy MP and Beckman, JS. (2006). Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc Natl Acad Sci U S A. 103(41):15038–15043. PMID: 17015830

Zhang Y, Vogel WK, McCullar JS, Greenwood JA, Filtz TM. (2006). Phospholipase C-beta3 and -beta1 form homodimers, but not heterodimers, through catalytic and carboxyl-terminal domains. Mol Pharmacol. 70(3):860-8. PMID: 16763092

Le UM, Yanasarn N, Löhr CV, Fischer KA, Cui Z. Tumor chemo-immunotherapy using gemcitabine and a synthetic dsRNA. Cancer Biol Ther. 2007 Dec 13;7(3) [Epub ahead of print] PMID: 18094613

Mata, JE, Dyal, LA, Slausson, M, Löhr, CV, Summerton, JE, Tyson, AR and Gustafson, SB. (2007). Tumor imaging using technicium-99m bound to pH sensitive peptides. Nanomedicine. 3(4):297-305. PMID: 17301838

Ishmael, JE, Löhr, CV, Fischer, KA and Kioussi, C. (2007). Localization of myosin II regulatory light chain in the cerebral vasculature. Acta Histochem. Oct 16 (Epub ahead of print) PMID: 17961638

Mathew LK, Sengupta S, Kawakami A, Andreasen EA, Löhr CV, Loynes, CA, Renshaw SA, Peterson RT, Tanguay RL. (2007). Unraveling tissue regeneration pathways using chemical genetics. J Biol Chem 282:35202-10 (E pub Sep 11, 2007) PMID: 17848559

Kioussi, C, Appu, M, Löhr, CV, Fischer, KA, Bajaj, G, Leid, M and Ishmael, JE. (2007). Co- expression of myosin II regulatory light chain and the NMDAR1 subunit in neonatal and adult mouse brain. Brain Res Bull 74:439-51 (Epub 2007 Aug 14) PMID: 17920452

Shaker DS, Sloat BR, Le UM, Löhr CV, Yanasarn N, Fischer KA, Cui Z. (2007). Immunization by application of DNA vaccine onto a skin area wherein the hair follicles have been induced into anagen-onset stage. Mol Ther15:2037-43 (Epub 2007 Aug 14) PMID: 17700542

Carter O, Dashwood RH, Wang R, Dashwood WM, Orner GA, Fischer KA, Löhr CV, Pereira CB, Bailey GS, Williams DE. (2007). Comparison of white tea, green tea, epigallocatechin-3-gallate, and caffeine as inhibitors of PhIP-induced colonic aberrant crypts. Nutr Cancer. 2007;58(1):60-5. PMID: 17571968

Indra, AK, Castaneda, E, Antal, MC, Jiang, M., Messaddeq, N., Meng, X., Loehr, CV, Gariglio, P., Kato, S., Wahli, W, Desvergne, B., Metzger, D. and Chambon, P. (2007). Malignant transformation of DMBA/TPA-induced papillomas and nevi in the skin of mice selectively lacking RXRα in epidermal keratinocytes. J Investigative Dermatol127:1250-60. PMID: 17301838

Andreasen, EA, Mathew, LK, Löhr, C, Hasson, R and Tanguay, RL. (2007). Aryl hydrocarbon receptor activation impairs extracellular matrix remodeling during zebrafish fin regeneration. Toxicol Sci 95:215-26. PMID: 17003102

Cope RB, Loehr C, Dashwood R, Kerkvliet NI. (2006). Ultraviolet radiation-induced non-melanoma skin cancer in the Crl:Skh:hr-1 hairless mouse: augmentation of tumor multiplicity by chlorophyllin and protection by indole-3-carbinol. Photochem Photobio Sci 5:499-507. PMID: 16685328.

Smith-Roe, SL, Löhr, CV, Bildfell, RJ, Fisher, KA, Hegan, DC, Glazer, PM and Buermeyer, AB. (2006). Induction of aberrant crypt foci in DNA mismatch repair-deficient mice by the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP). Cancer Lett 244:79-85 PMID: 16427736

Yu, Z, Mahadevan, B, Löhr, CV, Fischer, KA, Louderback, MA, Krueger, SK, Pereira, CB, Albershardt, DJ, Baird, WM, Bailey GS and Williams, DE. (2006). Indole-3-Carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon, dibenzo[a,l]pyrene. Carcinogenesis 27:2116-2123. PMID: 16704990