OREGON STATE UNIVERSITY
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Cell Imaging and Analysis (CIA) Facilities and Services Core

Mission
Access to state-of-the-art instrumentation and expertise in confocal microscopy, flow cytometry, cell sorting, and histopathology.

Background
Imaging is an indispensable mode of inquiry for researchers studying the mechanisms by which environmental toxins affect tissues, cells, and subcellular organelles. Recent advances such as fluorescent probes, green fluorescent protein (GFP), confocal optics, two-photon optics, and photoelectronic detectors have improved our ability to image complex tissues. They have enabled us to visualize single molecules and to monitor molecular interactions in live cells. Cost and complexity issues make it impractical for individual investigators to obtain state-of-the-art imaging technologies. Therefore, the need exists for an organized group of specialists with expertise in imaging technologies to acquire the instrumentation, master the applications, and help individual investigators apply the technology to answer specific research questions.

The below links provide detailed information on the technologies for cell imaging, flow cytometry and cell sorting and the pathological analysis of carcinogenetic studies.

Services

We support EHSC Investigators to accomplish these tasks:

  • Determine the damage resulting from exposure to environmental toxins and other treatments by providing expert pathological analysis from an experienced Veterinary Pathologist.
  • Acquire high-resolution images of zebrafish and other models by providing instrumentation, analysis software, infrastructure, and technical assistance.
  • Quantify the activation state of signal transduction pathways by developing advanced methodology using flow cytometry, microscopy, and mass spectrometry.
  • Isolate specific cell populations from cell culture, blood, and tissue by providing instrumentation and technical assistance.

Note: Any manuscript submitted for publication that contains data generated in these facilities should contain an acknowledgement of the services provided by the "Cell Imaging and Analysis Facilities and Services Core of the Environmental Health Sciences Center, Oregon State University, grant number P30 ES00210, National Institute of Environmental Health Sciences, National Institutes of Health."

2012 Publications

Lal, S., La Du, Jane, Tanguay, R.L., and Greenwood, J. A. (2012) Calpain 2 is required for the invasion of glioblastoma cells in the zebrafish brain microenvironment.  J. Neurosci. Res., 90, 769-81.  PMID: 22183788

Wang, R., Lohr, C.V., Fischer, K.A., Dashwood, W.M., Greenwood, J.A., Ho, E., Williams, D.E., Ashktorab, H., Dashwood, M.R., and Dashwood, R.H. (2012) Epigenetic inactivation of endothelin-2 and ET-3 in colon cancer.  Int. J. Cancer, 132, 1004-12.  PMID: 22865632

Smith, M., Boenzli, M., Hindagolla, V., Ding, J., Miller, J., Hutchison, J., Greenwood, J.A., Abeliovich, H., and Bakalinsky, A. (2012) Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity.  Appl. Environ. Microbiol., 79, 728-33.  PMID: 23144132

Campbell, A.L., Shih, H.P., Xu, J., Gross, M.K., Kioussi, C. (2012) Regulation of motility of myogenic cells in filling limb muscle anlagen by Pitx2.  PLoS One 7, 335822.  PMID 22558231

Rhoads T.W., Williams J.R., Lopez N.I., Morré J.T., Bradford C.S., Beckman J.S. (2012) Using Theoretical Protein Isotopic Distributions to Parse Small-Mass-Difference Post-Translational Modifications via Mass Spectrometry.  J Am Soc Mass Spectrom. 2012 Dec 18. [Epub ahead of print]  PMID: 23247967

O’Donnell, E.F., Kopparapu, P.R., Koch, D.C., Jang, H.S., Phillips, J.L., Tanguay, R.L., Kerkvliet, N.I., Kolluri, S.K.  (2012) The Aryl Hydrocarbon Receptor Mediates Leflunomide-Induced Growth Inhibition of Melanoma Cells. PLoS ONE 7(7): e40926.  PMID: 22815870

Rohlman, D., Pham, D., Yu, Z., Steppan, L., and Kerkvliet, N. (2012). Aryl hydrocarbon receptor-mediated perturbations in gene expression during early stages of CD4+ T-cell differentiation. Frontiers in Immunology 3, 223.  PMID: 22888330

Corsini, E., Oukka, M., Pieters, R., Kerkvliet, N.I., Ponce, R., Germolec, D.R. (2011) Alterations in regulatory T-cells: rediscovered pathways in immune-toxicology. J. Immunotoxicol. 8, 251-7. PMID: 21848365

Kerkvliet, N.I. (2012) TCDD: An environmental immunotoxicant reveals a novel pathway of immunoregulation – a 30-year odyssey.  Toxicol. Pathol. 40, 138-42. PMID: 22089840

Jason L. Seitchik, Jennifer C. Peeler, Michael T. Taylor, Melissa L. Blackman, Timothy W. Rhoads, Richard B. Cooley, Christian Refakis, Joseph M. Fox, and Ryan A. Mehl. Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific in Vivo Bioorthogonal Ligation with trans-Cyclooctenes. J. Am. Chem. Soc. 2012, 134(6), 2898-901.
Ryan A. Mehl. Engineered Unnatural Animals: Tools for Multicellular Biochemistry. ChemBioChem. 2012 13(2), 186-8.

2011 Publications

Corsini, E., Oukka, M., Pieters, R., Kerkvliet, N.I., Ponce, R., Germolec, D.R. (2011) Alterations in regulatory T-cells: rediscovered pathways in immune-toxicology. J. Immunotoxicol. [Epub ahead of print] PMID: 21848365

Kerkvliet, N.I. (2012) TCDD: An environmental immunotoxicant reveals a novel pathway of immunoregulation – a 30-year odyssey.  Toxicol. Pathol. [Epub ahead of print]. PMID: 22089840

Lal, S., La Du, Jane, Tanguay, R.L., and Greenwood, J. A. (2011) Calpain 2 is required for the invasion of glioblastoma cells in the zebrafish brain microenvironment.  J. Neurosci. Res., 2011 Dec 20. doi: 10.1002/jnr.22794. [Epub ahead of print] PMID: 22183788
 
Li Q, Luo C, Löhr CV, Dashwood RH. Activator protein-2α functions as a master regulator of multiple transcription factors in the mouse liver. Hepatol Res. 2011 Aug;41(8):776-83. doi: 10.1111/j.1872-034X.2011.00827.x. Epub 2011 Jun 17.
 
Ma, L., Greenwood, J.A., and Schachner, M. (2011) CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.  J. Neurosci. 31, 16781-91. PMID: 22090504

McDevitt, R.I., Ruaux, C.G., and Baltzer, W.I.  (2011) Influence of transfusion technique on survival of autologous red blood cells in the dog.  Journal of Veterinary Emergency and Critical Care, 21:209-216. PMID: 21631706

Saili K.S., Corvi M.M., Weber D.N., Patel A.U., Das S.R., Przybyla J., Anderson K.A., and Tanguay R.L. (2012) Neurodevelopmental low-dose bisphenol A exposure leads to early life-stage hyperactivity and learning deficits in adult zebrafish.  Toxicology 291(1-3):83-92. PMID: 22108044; PubMed Central PMCID: PMC3245816.

Shorey, L., Castro, D.J., Baird, W., Siddens, B., Lohr, C., Pereira, C., Waters, K., Corley, R.A., and Williams, D.E.  Transplacental carcinogenesis with dibenzo[def,p]chrysene (DBC): Timing of maternal exposures determines target tissue response in offspring. Cancer Lett. In press.  MFBSC cited ES03850

Wang, R., Dashwood, W.-M., Nian, H., Löhr, C.V., Fisher, K.A., Tsuchiya, N., Nakagama, H., Ashktorab, H., and Dashwood, R.H. (2011) NADPH oxidase overexpression in human colon cancers and rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Int J Cancer. 128(11), 2581–2590. PMCID: PMC3262595