Cell Imaging and Culture Facilities

	Jeffrey A. Greenwood, Ph.D., Director jeffrey.greenwood@orst.edu 541-737-4997

• Zeiss lSM 510 META Confocal Microscope

  The CIAFC operates a Zeiss LSM 510 META Confocal Microscope, which contains four lasers providing seven laser lines for imaging a wide variety of fluorescent probes. In addition, the META detector can separate highly overlapping spectra. An array of objectives is available to accommodate diverse sample types from cultured cells to animal or human tissue. The system contains a physiology incubation chamber for imaging live cells. The manufacturer designed the confocal system’s operating software for specialized applications such as fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET) in fixed and live cells. The Confocal Microscope resides in ALS2070, a low-vibration room near the cell culture rooms and Dr. Greenwood’s laboratory. 

• Zeiss Axiovert 100S Widefield Microscope

  In addition, the facility is equipped with a Zeiss Axiovert 100S widefield microscope for examining fixed or live cells via fluorescence, phase contrast, differential interference contrast, and interference reflection microscopy. The microscopy system contains a Photometrics CoolSNAP HQ CCD camera, a high-resolution digital camera that can image in the near-infrared. MetaMorph Imaging Software controls image acquisition and provides extensive analysis functions. 

• Eppendorf Semi-Automatic Microinjection System
  

  The facility contains an Eppendorf semi-automatic microinjection system for experiments that require rapid injection of DNA, protein, and other molecules into adherent cells. 

• Cell Culture Rooms
  

  The facility includes four cell culture rooms equipped with laminar-flow hoods, incubators, water baths, tabletop centrifuges, phase and fluorescence microscopes, refrigerators/freezers (4oC/-20oC/-85oC), and large-scale roller bottle/suspension culture equipment. One of the cell culture rooms and hood is approved for Biosafety Level 2 work. The facility also contains a KODAK Image Station 440CF, SpectraCount microplate reader, BioRad SmartSpec 3000, Beckman J2-21 centrifuge, Beckman J-6B centrifuge, autoclave, microfuge, chemical fume hoods, water purification system, microwave and baking oven, pan and analytical balances, pH meter, and sonicator. The core maintains cryogenic freezers for long-term storage of cells in liquid nitrogen. CIAFC staff maintains a computerized inventory of all cells to facilitate organization and retrieval of cell lines.

Staff

Tamara Fraley
Faculty Research Assistant
Department of Biochemistry and Biophysics

tamara.fraley@oregonstate.edu
541-737-3198