Aquatic Toxicology and Transgenics
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Robert L. Tanguay, Ph.D., Director
Phone: 541-737-6514 |
Learn more |
The Aquatic Toxicology and Transgenics Facility Core is unique worldwide as a facility equipped to conduct biomedical research using two of the most powerful aquatic research models, rainbow trout and zebrafish. It primarily consists of a self-contained fish hatchery and histopathology complex for rearing fish and for conducting tumor and toxicity studies. For the carcinogenesis and toxicity studies, investigators can chose several exposure routes, such as water-bath exposure of embryos or fry, dietary exposure, or embryo injection.
The goal of this core is to facilitate the short- and long-term experiments of center investigators by:
- Providing consultation regarding the feasibility of conducting biomedical studies in aquatic models;
- Providing high quality rainbow trout and zebrafish at multiple life stages;
- Providing technical core support including tank allocations, diet preparation and feeding;
- Providing assistance and in chemical exposure;
- Providing specific expertise in surgery and necropsy; and histopathological evaluation; and
- Work with the investigators in data acquisition and interpretation.
Facilities, Services and Equipment
Rainbow trout support: The research facility provides a complete, self-contained rainbow trout hatchery equipped to maintain and propagate Shasta strain rainbow trout, incubate and hatch artificially spawned eggs, and rear fry to varying experimental endpoints. It is also equipped to expose fish at various life stages to chemical carcinogens for either short-term or long-term experiments. Experimental fish are fed a reproducible, semi-purified diet into which toxins or other chemicals can be easily incorporated. This facility has the capability to conduct tumor studies (9-12 months) with up to 40,000 rainbow trout.
Zebrafish Support: The current zebrafish facility is composed of approximately 400 research tanks capable of holding up to 4,000 adult zebrafish for short and long-term studies. A significant expansion project is underway in which tank capacity will increase 10-fold. One of the unique advantages of zebrafish is the availability of hundreds of mutant and transgenic zebrafish with unique properties suited for specific experiments. This core allows our investigators access to any of these animals by providing a mechanism for their incorporation and propagation in the core. Currently, several wild-type zebrafish strains are maintained in the facility. In addition, a number of transgenic reporter lines are maintained which express GFP in specific cells and organs. In addition we have assembled the tools necessary to provide investigators access to transgenic approaches in zebrafish. Transient over expression (DNA and RNA) and antisense (modified oligonucleotides, morpholinos) repression technique are now routine.
| Facility Request Forms |
Facility Calendars |
Experiment Request Form |
Exposure Room |
Update Experiment Request Form |
Spawn Dates |
Service Request Form |
Trout Sampling |
Staff
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Eric Johnson, M.S., Fisheries/Marine Biologist Facility Manager Phone: 541-737-4389 Email: Eric.Johnson@oregonstate.edu Fax: 541-737-6074 Eric is in charge of the day-to-day operations of the facility and offers expertise in fish husbandry, chemical exposures of fish via several routes of administration, computer support for facility operation and data retrieval, fish dissection, laboratory instrument operation and maintenance and other functions. |
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Greg Gonnerman, B.S., Fisheries Biologist Assistant Facility Manager Phone: 541-737-4393 Email: Gman@oregonstate.edu Fax: 541-737-6074 Greg is responsible for diet preparation, feeding, spawning, egg, and fry care, maintenance of the laboratory, fish dissection and other necessary duties. |
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Sheila Cleveland, Histopathology Technician Phone: 541-737-4391 Email: Sheila.Cleveland@oregonstate.edu Fax: 541-737-6074 Sheila operates a complete histology preparatory laboratory, providing quality histology slides for verification of lesions produced in the long- and short-term experiments. |
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Cari Buchner, B.S., Fish Specialist Phone: 541-737-4392 Email: Cari.Buchner2@oregonstate.edu Fax: 541-737-6074 Cari works with rainbow trout, and her focus is the Toxic diet prep. The majority of her time is spent on feeding, husbandry, health monitoring, and when time allows, some facility maintenance projects. |
Publications
Harper, S.L., J.A. Dahl, B.L.S. Maddux, R.L. Tanguay and J.E. Hutchison. (2008) Proactively designing nanomaterials to enhance performance and minimize hazard. International Journal of Nanotechnology, in press
Usenko, Crystal Y., Harper, Stacey L., Tanguay, Robert L., Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish, Toxicology and Applied Pharmacology (2008), doi: 10.1016/j.taap.2007.12.030
Walter, RB, Timmins, GS, Tilton, SC, Orner, GO, Benninghoff, AD, Bailey, GS and Williams, DE (2008) Carcinogenesis Models: Focus on Xiphophorous and Rainbow Trout, in Oceans and Human Health, Elsevier Press, in press.
Benninghoff, A.D. and Williams, D.E. (2008) Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver. Toxicol Sci. 2008 Jan;101(1):65-80. Epub 2007 Sep 6. PMID: 17823450
Andreasen, E.A., Mathew, L., Loehr. C. Hasson, R. and Tanguay, R.L. (2007) Aryl hydrocarbon receptor activation impairs extracellular matrix remodeling during zebra fish fin regeneration. Toxicol Sci. 2007 Jan;95(1):215-26. Epub 2006 Sep 26. PMID: 17003102
*Evans, B.R., Karchner, S. I., Allan, L.L., Pollenz, R. S., Tanguay, R.L., Jenny, M.J., Sherr, D.H. and Hahn, M.E. Repression of aryl hydrocarbon receptor (AHR) signaling by AHR repressor: role of DNA binding and competition for AHR nuclear translocator. Mol Pharmacol. 2008 Feb;73(2):387-98. Epub 2007 Nov 13. PMID: 18000031
Friedman, M., McQuistan, T., Hendricks, J. and Bailey, G . Protective effect of dietary tomatine against dibenzo[a,l]pyrene (DBP)-induced liver and stomach tumors in rainbow trout. Mol Nutr Food Res. 2007 Dec;51(12):1485-91. PMID: 17979099
Higdon, J.V., Delage, B., Williams, D.E. and Dashwood, R.H. Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res. 2007 Mar;55(3):224-36. Epub 2007 Jan 25. PMID: 17317210
Isaacson, C. W., Usenko, C. Y., Tanguay, R. L., and Field, J. A. Quantification of fullerenes by LC/ESI-MS and its application to in vivo toxicity assays. Anal Chem 2007 Dec 1;79(23):9091-7. Epub 2007 Oct 27. PMID: 17963360
Mathew, L.K., Sengupta, S., Kawakami, A., Andreasen, E.A., Lohr, C.V., Loynes, C.A., Renshaw, S.A., Peterson, R.T., and Tanguay, R.L. (2007) Unraveling tissue regeneration pathways using chemical genetics. J Biol Chem. 2007 Nov 30;282(48):35202-10. Epub 2007 Sep 11. PMID: 17848559
Pratt, M.M., Reddy, A. P., Hendricks, J.D., Pereira, C., Kensler, T.W., and Bailey, G.S. (2007). The importance of carcinogen dose in chemoprevention studies: quantitative interrelationships between, dibenzo[a,l]pyrene dose, chlorophyllin dose, target organ DNA adduct biomarkers and final tumor outcome. Carcinogenesis. 2007 Mar;28(3):611-24. Epub 2006 Sep 14. PMID: 16973675
Simonich, M.T., McQuistan, T., Jubert, C., Pereira, C., Hendricks, J.D., Dashwood, R.H., Williams, D., and Bailey, G.S. (2007). Low-dose dietary chlorophyll inhibits multi-organ carcinogenesis in the rainbow trout. Food Chem Toxicol. 2007 Nov 4; [Epub ahead of print] PMID: 18069110
Tilton, S.C., Hendricks, J.D., Orner, G.A., Pereira, C.B., Bailey, G.S., and Williams, D.E. (2007) Gene expression analysis during tumor enhancement by the dietary phytochemical, 3,3'-diindolylmethane, in rainbow trout. Carcinogenesis. 2007 Jul;28(7):1589-98. Epub 2007 Feb 1. PMID: 17272308
Usenko, C.Y., Harper, S.L., and Tanguay, R.L. (2007) In vivo evaluation of carbon fullerene toxicity using embryonic zebrafish. Carbon 45: 1891–1898 (paper copy provided).
Tilton, F., Ladu, J. K., Vue, M., and Tanguay, R.L. (2006) Dithiocarbamates have a common toxic effect on zebrafish body axis formation. Toxicol Appl Pharmacol 216(1): 55-68. PMID: 16797628
Reimers, M. J., Ladu, J.K., Periera C. B., Giovanini, J. and Tanguay, R.L. (2006) Ethanol-dependent toxicity in zebrafish is partially attenuated by antioxidants. Neurotoxicol Teratol. 28(4): p. 497-508. PMID: 16904866
Prasch, A.L. Tanguay R.L., Mehta, V., Heideman W., and Peterson R.E. (2006) Identification of zebrafish ARNT1 homologs: 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in the developing zebrafish requires ARNT1. Mol Pharmacol. 2006 Mar;69(3):776-87. Epub 2005 Nov 23. MID: 16306231
